Student Name __________________________ Date____________LAB REPORT EVALUATION MATRIX (Biology 156)GOOD AVERAGE POOR NOT PRESENT(BUT NEEDED)ABSTRACT to include the following (10 pts)250 words or less _______ ________ _______ _______problem investigated _______ ________ _______ _______methods _______ ________ _______ _______major results _______ ________ _______ _______conclusions _______ ________ _______ _______significance of findings _______ ________ _______ _______TOTAL _______INTRODUCTION to include the following (20 pts)sufficient background info on organism/systemwith citations _______ ________ _______ _______problem clearly defined _______ ________ _______ _______purpose and goals _______ ________ _______ _______hypothesis/prediction _______ ________ _______ _______TOTAL _______MATERIALS AND METHODS to include the following (20 pts)summary of process _______ ________ _______ _______purpose of a procedure _______ ________ _______ _______no excess mundane details _______ ________ _______ _______TOTAL _______RESULTS to include the following (15 pts)Tables and graphs clearly labeled _______ ________ _______ _______Narrative includes summary tables and graphs _______ ________ _______ _______TOTAL _______DISCUSSION to include the following (20 pts)interpretation of results _______ ________ _______ _______Explanation of significance of results _______ ________ _______ _______has related discussion of results to the goals,hypothesis and purpose of experiment, includingconclusions _______ ________ _______ _______includes citations _______ ________ _______ _______TOTAL _______LITERATURE CITED to include the following (5 pts)listed alphabetically _______ ________ _______ _______cited properly within narrative of paper _______ ________ _______ _______correct format of citation _______ ________ _______ _______TOTAL _______WRITTEN EFFECTIVELY to include the following (10 pts)written clearly _______ ________ _______ _______Title page _______ ________ _______ _______use of metric system _______ ________ _______ _______each paragraph conveys a single major idea and hastopic sentence at beginning _______ ________ _______ _______pages numbered _______ ________ _______ _______avoidance of I and me statements _______ ________ _______ _______TOTAL _______Lab Report Grade ________Using Transpirometers Must be put in your own wordPreparing the Plant (this can be done hours before the experiment is to be conducted):1. Select a plant that has a section of stem that is of slightly larger diameter than the tanrubber tubing attached to the pipette tip.2. Cut the plant off at its base, below where the diameter is slightly larger than the rubbertubing. Immerse cute end in pan of water.3. Immerse the pipette with rubber tubing attached in the pan of water to remove all air fromthe pipette and tubing. This may require pinching and squeezing the tubing. All airbubbles must be removed.4. With the stem submerged, cut the plant again at the point where the stem is slightly largerthan the rubbing tubing. Keep the cut stem under water at all times.5. Slide the rubber tubing over the cut stem.6. Hold the plant and tubing out the water and check for leaks. If air percolates into the tipof the pipette right away then there is a leak. If there is a leak, resubmerge and try slidingthe tubing onto the stem farther.Preparing the Apparatus:1. Flush the system by opening the screw valve below the reservoir. Flush until no bubblesleave the micropipette then clamp off the micropipette using the spring clamp. Continueflushing until no bubbles are present in the line where the plant will be inserted.2. Insert the plant and pipette combination into the tube. Wrap a paper towel around thestem of the plant and insert the plant into the open clamp. Clamp just enough to hold theplant upright.3. Open the spring clamp on the line leading to the micropipette.4. Open the screw valve on the reservoir just enough for water to drip from the micropipetteand then close the screw valve. Blot the drop from the tip of the pipette.5. If the leaves of the plant are very wet, gently blot them dry.6. Transpiration should be noticeable within a few minutes as water is drawn from themicropipette.Measuring Transpiration Rates:1. The micropipette is marked at the 100 μL point. Students can measure the length to themark (usually about 88 mm) and then calculate a conversion factor (88 mm/100 μL).Using the conversion factor they can calculate the volume transpired per unit time.2. Factors that may affect transpiration rates are light, heat, and wind. However, exposureto extremes of heat or wind may cause the plant to close its stomates rather than transpiremore. This might be something to try at the end of a lab period.